substrate affinity

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• Affinity chromatography — is a method of separating biochemical mixtures and based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Contents 1 Uses 2 Principle 3 Batch and column setup …   Wikipedia

• affinity chromatography — n chromatography in which a macromolecule (as a protein) is isolated and purified by passing it in solution through a column that has been treated with a substance having a ligand for which the macromolecule has an affinity that causes it to be… …   Medical dictionary

• Affinity label — is a molecule that is similar in structure to a particular substrate for a specific enzyme. It is considered to be a class of irreversible inhibitors. These molecules covalently modify active site residues in order to elucidate the structure of… …   Wikipedia

• affinity chromatography — Chromatography in which the immobile phase (bed material) has a specific biological affinity for the substance to be separated or isolated, such as the affinity of an antibody for its antigen, or an enzyme for a substrate analogue …   Dictionary of molecular biology

• substrate — 1. The substance acted upon and changed by an enzyme; the reactant considered to be attacked in a chemical reaction. 2. The base on which an organism lives or grows; e.g., the s. on which microorganisms and cells grow in cell culture. [L. sub… …   Medical dictionary

• affinity — An expression of the strength of interaction between two entities, eg. between receptor and ligand or between enzyme and substrate. The affinity is usually characterized by the equilibrium constant association constant or dissociation constant… …   Dictionary of molecular biology

• Allosteric regulation — In biochemistry, allosteric regulation is the regulation of an enzyme or other protein by binding an effector molecule at the protein s allosteric site (that is, a site other than the protein s active site). Effectors that enhance the protein s… …   Wikipedia

• PDE3 — phosphodiesterase 3A, cGMP inhibited Identifiers Symbol PDE3A Entrez 5139 HUGO …   Wikipedia

• Receptor (biochemistry) — For other uses, see Receptor (disambiguation). In biochemistry, a receptor is a molecule found on the surface of a cell, which receives specific chemical signals from neighbouring cells or the wider environment within an organism. These signals… …   Wikipedia

• SLC13A3 — Solute carrier family 13 (sodium dependent dicarboxylate transporter), member 3, also known as SLC13A3, is a human gene.cite web | title = Entrez Gene: SLC13A3 solute carrier family 13 (sodium dependent dicarboxylate transporter), member 3| url …   Wikipedia

• Dibucaine number — Dibucaine, also known as cinchocaine, is an amino amide local anesthetic. It is commonly used as an agent for central neuraxial anesthesia in Great Britain. When administered to humans intravenously, it is capable of inhibiting the plasma… …   Wikipedia